I don't think theres' a difference. I just did those last month and didn't always remember to do it first thing! I'd just hold my pee for a little bit longer if I could and do it then.
I used wondfos around 1-5 most days with a pee hold. LH hormone typically spikes in the early morning, so you want to avoid testing after you wake up and let the hormones build up in the urine to get a good reading.
I used wondfos around 1-5 most days with a pee hold. LH hormone typically spikes in the early morning, so you want to avoid testing after you wake up and let the hormones build up in the urine to get a good reading.
I would still test at 5.
This is also what my RE said- they recommend that I test at night (after holding pee for 3 hours)